Related Experiment Video
Updated: Jul 30, 2025
06:58
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
7.1K
Genome Features of Probiotic Bifidobacteria Determining Their Strain-Specific Properties
A G Tochilina1, I V Belova2, T N Ilyicheva3
1Senior Researcher, Laboratory of Human Microbiome and Means of Its Correction; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Rospotrebnadzor (Russian Federal Consumer Rights Protection and Human Health Control Service), 71 Malaya Yamskaya St., Nizhny Novgorod, 603950, Russia; Associate Professor, Department of Epidemiology, Microbiology and Evidence-Based Medicine; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Sovremennye Tekhnologii V Meditsine
|May 14, 2023
Summary
Genome analysis of Bifidobacterium strains revealed key genetic features. Bifidobacterium bifidum 791 demonstrated significant antiviral activity against influenza A, linked to bacteriocin synthesis.
Area of Science:
- Microbiology
- Genomics
- Virology
Background:
- Probiotic bacteria, such as Bifidobacterium species, are known for their health benefits.
- Understanding the genetic basis of probiotic properties can enhance their application.
- Antiviral properties of probiotics are of increasing interest for therapeutic development.
Purpose of the Study:
- To analyze the complete genome sequences of three probiotic Bifidobacterium strains: B. longum 379, B. bifidum 1, and B. bifidum 791.
- To identify specific genetic determinants responsible for their unique characteristics.
- To evaluate the antiviral activity of these strains against influenza A viruses.
Main Methods:
- Whole genome sequencing was performed using the MiSeq platform.
- Genome annotation was conducted using Prokka and RAST.
- Genetic determinants were identified using ResFinder, PathogenFinder, PlasmidFinder, RAST, and Bagel 4.
- Antiviral activity was assessed in vitro using MDCK cells and influenza A virus strains (H1N1 pdm09 and H5N6).
Main Results:
- All strains possess genes for plant carbohydrate utilization.
- B. longum 379 and B. bifidum 791 genomes contain genes for tryptophan and folic acid synthesis.
- B. bifidum 791 genome uniquely harbors determinants for thermostable type I bacteriocins (flavucin and lasso peptide).
- B. bifidum 791 exhibited significant antiviral activity, inhibiting influenza A replication and reproduction.
Conclusions:
- Genome analysis elucidated strain-specific properties of B. longum 379, B. bifidum 1, and B. bifidum 791.
- The presence of tryptophan and folic acid synthesis genes in B. longum 379 and B. bifidum 791 may contribute to their beneficial effects, including mood regulation.
- The genetic determinants for bacteriocin production in B. bifidum 791 directly correlate with its potent antiviral activity against influenza A.
Related Concept Videos
01:27Bacterial Phylum Firmicutes
64
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
64
01:30Bacterial Phylum Actinobacteria
40
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
40
01:26Bacterial Phylum Proteobacteria
51
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
51
01:29Bacterial Flora of the Large Intestine
538
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
538
00:46Genomic DNA in Prokaryotes
44.1K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.1K
01:26Bacterial Phylum Verrucomicrobiota
46
The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
46