Three-dimensional Culture Using Atelocollagen Sponge and Self-assembling Peptide Hydrogel

This study aimed to assess the combined application of two biomaterials, a selfassembling peptide hydrogel (SPH) and an atelocollagen sponge (ACS). The ACS was combined with SPH (PuraMatrix^Ⓡ or PanaceaGel^Ⓡ) and its osteogenic effects on mouse osteoblastic cell line MC3T3 then evaluated. Each type of SPH was successfully incorporated into the ACS. The MC3T3 cells showed uniform distribution within the scaffold. No necrotic cells were observed throughout the experimental procedures. When the SPH was combined with the ACS, the MC3T3 cells differentiated toward the osteo-lineage, expressing Alp, Runx2, Osx, Bsp, and Oc. PanaceaGel^Ⓡ exhibited a stronger osteogenic effect on the cells than PuraMatrix^Ⓡ.