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Updated: Jul 30, 2025

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y-mtPTM: Yeast mitochondrial posttranslational modification database.

Bronislava Brejová1, Veronika Vozáriková2, Ivan Agarský1

  • 1Department of Computer Science, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Bratislava 842 48, Slovakia.

Genetics
|May 15, 2023
PubMed
Summary

Researchers created the yeast mitochondrial posttranslational modification (y-mtPTM) database to consolidate information on protein modifications. This resource aids in understanding the functional roles of posttranslational modifications in yeast mitochondria.

Keywords:
acetylationdatabasemitochondriaphosphorylationposttranslational modificationssuccinylationyeast

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Posttranslational modifications (PTMs) increase proteome complexity, creating diverse protein isoforms.
  • Mitochondrial PTMs are crucial for cellular functions but less studied than in other compartments.
  • Existing data on mitochondrial PTMs are fragmented across multiple datasets.

Purpose of the Study:

  • To integrate and centralize information on posttranslational modifications in the yeast Saccharomyces cerevisiae mitochondrial proteome.
  • To develop a comprehensive database for yeast mitochondrial PTMs.
  • To facilitate research on the functional significance of mitochondrial protein modifications.

Main Methods:

  • Utilized mass spectrometry to identify and characterize PTMs on mitochondrial proteins.
  • Compiled data from various sources to create a unified database.
  • Developed the yeast mitochondrial posttranslational modification (y-mtPTM) database.

Main Results:

  • The y-mtPTM database catalogs nearly 20,000 modified positions on yeast mitochondrial proteins.
  • Approximately 20 different types of PTMs are documented, with phosphorylation, succinylation, acetylation, and ubiquitylation being most common.
  • The database provides details on modified residues, positions (primary and 3D structure), and source references.

Conclusions:

  • The y-mtPTM database serves as a centralized resource for yeast mitochondrial PTM research.
  • It enables researchers to investigate the functional roles of PTMs in mitochondrial proteins.
  • This integrated platform supports deeper exploration of mitochondrial biology and PTMs.