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Related Experiment Video

Updated: Jul 30, 2025

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods
03:39

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

Published on: April 28, 2023

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A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods.

Qing Liu1, Lili Zhang2, Rongxiang Fang3

  • 1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences; University of Chinese Academy of Sciences.

Journal of Visualized Experiments : Jove
|May 15, 2023
PubMed
Summary

Researchers developed a simple method to collect hemolymph from small arthropods like the small brown planthopper (SBPH). This technique aids in studying arboviruses and vector-virus interactions, crucial for understanding disease transmission.

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Last Updated: Jul 30, 2025

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Area of Science:

  • Entomology
  • Virology
  • Molecular Biology

Background:

  • Arthropods transmit significant arboviruses via hemolymph.
  • Effective hemolymph collection is vital for studying virus-vector interactions.
  • Existing methods may be insufficient for small arthropods.

Purpose of the Study:

  • To introduce a novel, simple, and quantitative method for hemolymph collection from small arthropods.
  • To establish a reliable protocol using Laodelphax striatellus (small brown planthopper, SBPH) as a model.
  • To facilitate research on rice stripe virus (RSV) and other arboviruses.

Main Methods:

  • Utilized frozen arthropods (Laodelphax striatellus).
  • Gently detached a leg using fine-tipped tweezers.
  • Collected exuded hemolymph using a capillary micropipette and pipette bulb, leveraging capillary forces.

Main Results:

  • Successfully demonstrated a quantitative hemolymph collection technique.
  • The method is efficient and suitable for small arthropods.
  • Collected hemolymph is ready for buffer dissolution and further analysis.

Conclusions:

  • The described method provides a valuable tool for quantitative hemolymph collection in small arthropods.
  • This technique enhances the study of arboviruses and their interactions with vectors.
  • It offers a practical approach for entomological and virological research.