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Updated: Jul 30, 2025
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
In Vitro Biochemical Characterization of Excised Macrocyclizing Thioesterase Domains from Non-ribosomal Peptide
Jordan T Brazeau-Henrie1, André R Paquette1, Christopher N Boddy2
1Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada.
Abstract:
Characterization of thioesterases (TEs) is an important step in understanding natural product biosynthesis. Studying non-ribosomal peptide synthetase (NRPS) TEs presents a unique set of challenges with specific cloning and expression issues as well as the challenging synthesis of the thioester peptides substrate required for characterization of the TE. In this method, we describe the cloning and expression of NRPS TEs, the synthesis of thioester peptides, and the in vitro biochemical characterization of the enzyme.
