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Identification and characterization of BmNPV Bm5 protein required for the formation of nuclear vesicle structures

Xiangshuo Kong1,2, Guanping Chen1,2, Jiale Li1,2

  • 1College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.

The Journal of General Virology
|May 15, 2023
PubMed

Insights

Bombyx mori nucleopolyhedrovirus (BmNPV) Bm5 protein drives nuclear membrane invagination, impacting viral particle production. Nuclear vesicles are crucial for occlusion-derived virion (ODV) formation during baculovirus infection.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Baculovirus infections, specifically Bombyx mori nucleopolyhedrovirus (BmNPV), induce unique nuclear structures.
  • Intranuclear lipid accumulation, mediated by the Bm5 protein, is implicated in nuclear vesicle formation.
  • The precise role of Bm5 protein in baculovirus-induced nuclear vesicles remains incompletely understood.

Purpose of the Study:

  • To elucidate the relationship between BmNPV-induced nuclear vesicles and the Bm5 protein.
  • To investigate the function of nuclear vesicle-like structures in the baculovirus replication cycle.
  • To understand the mechanism of nuclear membrane invagination during BmNPV infection.

Main Methods:

  • Observation of nuclear vesicle-like structures formation and Bm5 protein localization during BmNPV infection.
  • Analysis of the impact of Bm5 protein on nuclear vesicle invagination and viral production.
  • Investigation of the role of ODV major envelope proteins (P74, Bm14) in relation to nuclear vesicles.
  • Examination of the involvement of nuclear F-actin in vesicle scission.

Main Results:

  • BmNPV Bm5 protein actively participates in the invagination of nuclear vesicle-like structures.
  • This invagination process influences the production of occlusion-derived virion (ODV) and occlusion body (OB).
  • Nuclear vesicle formation is not essential for the transport or recruitment of key ODV envelope proteins like P74 and Bm14.
  • Nuclear F-actin appears to provide mechanical force for the scission of large vesicle-like structures from the nuclear membrane.

Conclusions:

  • A novel pathway involving BmNPV Bm5 protein-induced nuclear membrane invagination has been identified.
  • Nuclear vesicle-like structures play a significant role in the production of ODV.
  • The study clarifies the function of nuclear vesicles in baculovirus replication and the role of Bm5 protein.
Keywords:
baculovirusnuclear actinnuclear membranenuclear vesicles

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