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Related Experiment Video

Updated: Jul 30, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation.

Pouya Sharbati^1,2, Abdolali K Sadaghiani^1,2, Ali Koşar^1,2

¹Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, Turkey.

|May 15, 2023

Summary

This summary is machine-generated.

This study presents a novel dielectrophoretic microfluidic device for precise multi-particle and cell separation. The device achieves 95.5% efficiency, offering a versatile platform for various biological applications.

Keywords:

3D electrodes blood cells dielectrophoresis electrochemical cells lab-on-a-chip

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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Area of Science:

Biotechnology
Microfluidics
Cell Separation

Background:

Accurate separation of diverse biological particles is crucial for research and diagnostics.
Existing methods often face limitations in handling multiple particle types simultaneously.

Purpose of the Study:

To develop and evaluate a new dielectrophoretic microfluidic device for high-efficiency, multi-type particle and cell separation.
To optimize device parameters for separating various biological entities, including red blood cells, T-cells, U937-MC cells, and bacteria.

Main Methods:

Utilized a microfluidic device with cylindrical and sidewall 3D electrodes for dielectrophoresis-based separation.
Employed red blood cells, T-cells, U937-MC cells, and Clostridium difficile bacteria as test subjects.
Optimized electrode geometry (200 µm sidewall electrodes) and applied voltages for distinct separation steps.

Main Results:

Achieved accurate separation of multiple bioparticle types in a two-step process.
Demonstrated optimal performance at 6 Vp-p (3D electrodes) and specific voltages for sidewall electrodes (20 Vp-p and 11 Vp-p).
Reached a maximum separation efficiency of 95.5% for multi-type particle separation without exceeding the cell electroporation threshold.

Conclusions:

The developed dielectrophoretic microfluidic device enables precise and efficient separation of multiple particle and cell types.
The device shows high accuracy and versatility, adaptable to a wide range of biological particles.
This technology holds significant potential for applications in cell sorting, diagnostics, and bioprocessing.