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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Jul 30, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Enzyme-Based Biosensors and Their Applications.

Yu-Fan Fan¹, Zhao-Bin Guo¹, Guang-Bo Ge¹

¹Shanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

|May 15, 2023

Summary

This summary is machine-generated.

Enzymes are vital biomacromolecules regulating cell metabolism and homeostasis. Their diverse catalytic functions are crucial for maintaining life processes in organisms.

Area of Science:

Biochemistry
Molecular Biology
Enzymology

Background:

Enzymes are essential biomacromolecules with critical roles in biological systems.

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Last Updated: Jul 30, 2025

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They act as catalysts, regulating cellular metabolism and maintaining homeostasis.

Understanding enzyme function is key to comprehending life processes.