Related Experiment Video

Updated: Jul 30, 2025

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
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A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

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Triptolide alleviates collagen-induced arthritis in mice by modulating Treg/Th17 imbalance through the JAK/PTEN-STAT3

Yao Huang1, Xin Ba1, Hui Wang2

  • 1Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Basic & Clinical Pharmacology & Toxicology
|May 15, 2023
PubMed
Abstract

Insights

Triptolide (TP) effectively reduces inflammation and bone damage in collagen-induced arthritis (CIA) mice by regulating T cell balance and inhibiting the JAK/PTEN-STAT3 pathway.

Area of Science:

  • Immunology
  • Pharmacology
  • Rheumatology

Background:

  • Collagen-induced arthritis (CIA) is a mouse model for rheumatoid arthritis.
  • Investigating novel therapeutic agents for CIA is crucial.

Purpose of the Study:

  • To evaluate the therapeutic effects of triptolide (TP) in CIA mice.
  • To elucidate the underlying mechanisms of TP's action.

Main Methods:

  • CIA mice were treated with TP for 35 days.
  • Assessed joint swelling, bone destruction, serum markers, and immune cell populations (Treg, Th1, Th17).
  • Utilized molecular techniques including flow cytometry, RT-qPCR, western blotting, immunofluorescence, and reverse docking to analyze the JAK/PTEN-STAT3 pathway.

Main Results:

  • TP significantly reduced joint swelling, bone destruction, and systemic inflammation.
  • TP treatment corrected the Treg/Th17 cell imbalance in CIA mice.
  • TP inhibited the JAK/PTEN-STAT3 signaling pathway activation in vivo and in vitro.

Conclusions:

  • Triptolide demonstrates significant anti-inflammatory and bone-protective effects in CIA mice.
  • TP's mechanism involves modulating Treg/Th17 cell balance via the JAK/PTEN-STAT3 pathway.
Keywords:
STAT3Th17 cellsTreg cellscollagen-induced arthritisreverse dockingtriptolide (TP)

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