Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of TIMP1, Transgelin-2, and Procalcitonin as Protein Biomarkers for Diagnosis and Prognosis of Bloodstream Infection in a Critically Ill Cohort.

The journal of applied laboratory medicine·2026
Same author

Inter-Instrument Imprecision Complements Bias in Evaluating LDL-C Method Performance.

Clinical chemistry·2026
Same author

Evaluation of the Impact of a Pathology Trainee-Driven Manual Review Process for Reference Testing at a Large Academic Center.

The journal of applied laboratory medicine·2026
Same author

From Diagnosis to Delivery: A Look at the Continuing Gap in Maternal Testing.

Clinical chemistry·2025
Same author

Evaluation of three NT-proBNP assays for heart failure.

Clinical biochemistry·2025
Same author

Breaking Barriers in Biosensing: Real-Time Continuous Protein Detection in Interstitial Fluid.

Clinical chemistry·2025
Same journal

Benchmarking Institutional Support for Point-of-Care Testing Programs: Scale, Staffing, and Operational Challenges.

The journal of applied laboratory medicine·2026
Same journal

Hidden Figures of DNA. 1. June (Broomhead) Lindsey.

The journal of applied laboratory medicine·2026
Same journal

The Emerging Role of Blood-Based Biomarkers in Predicting Alzheimer's Disease.

The journal of applied laboratory medicine·2026
Same journal

Healthcare Excellence in 2026: An Unprecedented Sweep for Global Growth Economies.

The journal of applied laboratory medicine·2026
Same journal

ADLM Guidance Document on Incorporating Gender Diversity in Pathology and Laboratory Medicine.

The journal of applied laboratory medicine·2026
Same journal

Leveraging an Explainable Machine Learning Model for Early Identification of Acute Kidney Injury: A Retrospective Study.

The journal of applied laboratory medicine·2026
See all related articles

Related Experiment Video

Updated: Jul 30, 2025

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

20.9K

Method Comparison and Workflow Differences Using the Same Free Light Chain Assay on 2 Analyzer Platforms.

Catherine L Omosule1, Karl G Hock1, Claire Dalton1

  • 1Department of Pathology & Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, United States.

The Journal of Applied Laboratory Medicine
|May 15, 2023
PubMed
Summary
This summary is machine-generated.

The Freelite assay demonstrates comparable analytical performance for serum free light chains (sFLC) on both Optilite and cobas 8000 analyzers. The Optilite system offers reduced reagent use and faster turnaround times, improving workflow efficiency for diagnosing plasma cell dyscrasias.

More Related Videos

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

10.8K
Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

16.8K

Related Experiment Videos

Last Updated: Jul 30, 2025

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

20.9K
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

10.8K
Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

16.8K

Area of Science:

  • Clinical Chemistry
  • Immunodiagnostics
  • Hematology

Background:

  • Serum immunoglobulin free light chains (sFLC) are critical biomarkers for diagnosing and monitoring plasma cell dyscrasias (PCDs).
  • The Freelite assay is a key method for quantifying sFLC levels in patient serum.
  • Evaluating different analytical platforms is essential for optimizing diagnostic workflows.

Purpose of the Study:

  • To compare the analytical performance of the Freelite assay on two different analyzer platforms: Roche cobas 8000 and Optilite.
  • To evaluate workflow differences, including turnaround time (TAT) and reagent consumption, between the two analyzers.

Main Methods:

  • sFLC concentrations were measured in 306 fresh serum specimens (cohort A) and 48 frozen specimens (cohort B) using Freelite κ and λ assays.
  • Specimens were analyzed on both Roche cobas 8000 and Optilite analyzers.
  • Performance was assessed using Deming regression, and workflow was evaluated by TAT and reagent usage.

Main Results:

  • Deming regression showed comparable analytical performance for sFLCκ and sFLCλ between the Optilite and cobas 8000 analyzers.
  • The Optilite analyzer demonstrated a significantly lower proportion of specimens with TAT >60 min (0.33% vs. 8%) compared to the cobas 8000 (P < 0.001).
  • The Optilite required fewer reagents for both sFLCκ (49 fewer tests, P < 0.001) and sFLCλ (12 fewer tests, P = 0.016) assays.

Conclusions:

  • The Freelite assays exhibit comparable analytical performance on both Optilite and cobas 8000 analyzers.
  • The Optilite system offers workflow advantages, including reduced reagent consumption and slightly decreased TAT.
  • The Optilite eliminates the need for manual dilutions for samples with high sFLC concentrations (>20 mg/dL).