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Related Experiment Video

Updated: Jul 30, 2025

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
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Optimizing the protocol for retinal vascular permeability mapping from fluorescein videoangiography data.

Elif Kayaalp Nalbant1, Laasya Annepureddy1, Shaoxian Hu1

  • 1Biomedical Engineering, Illinois Institute of Technology, Chicago, USA 60616.

Proceedings of Spie--The International Society for Optical Engineering
|May 15, 2023
PubMed
Summary

This study presents a clinical workflow to measure retinal vascular permeability and blood flow using fluorescein videoangiography. This method offers a new way to detect eye diseases early by assessing vascular health.

Keywords:
fluorescein videoangiographymathematical modelretinopathy

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Related Experiment Videos

Last Updated: Jul 30, 2025

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Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Vascular Biology

Background:

  • An intact blood-retinal barrier (BRB) is essential for retinal function.
  • Impaired vascular permeability is linked to various eye diseases.
  • Current methods lack direct clinical measures of vascular permeability.

Purpose of the Study:

  • To develop and validate a clinical workflow for quantifying retinal vascular permeability.
  • To measure volumetric blood flow using fluorescein videoangiography data.
  • To establish a method for early disease detection through vascular assessment.

Main Methods:

  • Developed a complete clinical workflow utilizing fluorescein videoangiography.
  • Quantified vascular permeability and volumetric blood flow from imaging data.
  • Optimized the protocol by adjusting frame rate for high-resolution imaging and error reduction.
  • Validated the workflow through realistic simulations.

Main Results:

  • Successfully detailed a clinical workflow for measuring vascular permeability and blood flow.
  • Demonstrated the feasibility of quantifying these parameters from fluorescein videoangiography.
  • Optimized imaging parameters for improved accuracy and resolution.

Conclusions:

  • The developed clinical workflow provides a direct method to measure retinal vascular permeability and blood flow.
  • This approach holds potential for the early detection of eye diseases.
  • Further clinical translation is supported by validation through simulations.