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Acute toxicity study of onion peel-derived gold nano-bioconjugate
Kabyashree Phukan1,2, Himangshu Sarma3, Rajlakshmi Devi3
1Material Nanochemistry Laboratory, Physical Sciences Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Garchuk, Guwahati, 781035 India.
Abstract:
The acute anti-inflammatory activity of onion peel-derived gold nano-bioconjugate was already established earlier. The current study was aimed to investigate the acute oral toxicity of onion peel-derived gold nano-bioconjugate (GNBC) for safe therapeutic utilization in vivo. The acute toxicity study was carried out in female mice for 15 days and showed no mortality and any abnormal complications. The lethal dose (LD50) was evaluated and found to be higher than 2000 mg/kg. After 15 days, animals were euthanized and hematological, and biochemical analyses were performed. In all hematological and biochemical assays, treated animals did not show significant toxicity when compared to the control group. The body weight, behavior, and histopathological studies showed that GNBC is nontoxic. Thereby, the results suggest that onion peel-derived gold nano-bioconjugate GNBC can be utilized for therapeutic applications in vivo.
Insights
Onion peel-derived gold nano-bioconjugate (GNBC) shows no toxicity in mice during a 15-day study. This research confirms GNBC
Area of Science:
- Nanomedicine
- Toxicology
- Bioconjugate Chemistry
Background:
- The anti-inflammatory effects of onion peel-derived gold nano-bioconjugate (GNBC) have been previously established.
- Assessing the in vivo toxicity of GNBC is crucial for its safe therapeutic application.
Purpose of the Study:
- To evaluate the acute oral toxicity of onion peel-derived gold nano-bioconjugate (GNBC) in a preclinical setting.
- To determine the safety profile of GNBC for potential therapeutic use.
Main Methods:
- Acute oral toxicity study conducted in female mice over 15 days.
- Assessment of mortality, clinical signs, body weight, hematological parameters, biochemical assays, and histopathological examination.
- Determination of the lethal dose (LD50).
Main Results:
- No mortality or abnormal complications were observed in mice treated with GNBC.
- The lethal dose (LD50) was determined to be greater than 2000 mg/kg.
- Hematological, biochemical, body weight, behavioral, and histopathological analyses indicated no significant toxicity.
Conclusions:
- Onion peel-derived gold nano-bioconjugate (GNBC) demonstrates a favorable safety profile.
- GNBC is deemed safe for therapeutic applications in vivo based on acute toxicity assessments.
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