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A method for the ultrastructural examination of cell monolayers cultured in plastic microtitre plates
Clinical and Experimental Immunology
|January 1, 1979
Abstract:
A method is presented by which cells growing as monolayers cultured in microtitre plates can be embedded for electron microscopy. The technique has the following advantages: numerous specimens may be prepared with relatively small numbers of cells, cell-cell interactions remain undisturbed and may be enumerated, and morphology can be studied under circumstances identical with those used in cell-mediated cytotoxicity assays.
Insights
This study introduces a new method for embedding cells cultured in microtitre plates for electron microscopy. This technique preserves cell interactions and allows for efficient specimen preparation for cell morphology studies.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Electron microscopy requires specialized sample preparation.
- Studying cell-cell interactions in vitro is crucial for understanding biological processes.
- Existing methods may not preserve cellular architecture or interactions effectively.
Purpose of the Study:
- To present a novel method for embedding monolayer cell cultures for electron microscopy.
- To highlight the advantages of this technique for cell biology research.
Main Methods:
- Embedding cells grown as monolayers in microtitre plates.
- Utilizing a technique compatible with standard cell-mediated cytotoxicity assays.
Main Results:
- Enables preparation of numerous specimens from small cell numbers.
- Preserves undisturbed cell-cell interactions for enumeration.
- Allows morphological studies under conditions mimicking functional assays.
Conclusions:
- The presented method offers an efficient and effective way to prepare cells for electron microscopy.
- This technique facilitates detailed analysis of cell morphology and interactions in a biologically relevant context.