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Related Experiment Videos

Protoheme-carbon monoxide geminate kinetics.

M C Marden, E S Hazard, Q H Gibson

    Biochemistry
    |May 20, 1986
    PubMed
    Summary

    This study measured the recombination rate of carbon monoxide (CO) to protoheme after laser photolysis. The geminate recombination rate constant was found to be 3 X 10^9/s, showing weak dependence on temperature and viscosity.

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    Biochemistry·2007

    Area of Science:

    • Biophysical chemistry
    • Photochemistry
    • Chemical kinetics

    Background:

    • Carbon monoxide (CO) binding to heme proteins is crucial for various biological processes.
    • Understanding CO recombination kinetics provides insights into protein dynamics and ligand escape mechanisms.

    Purpose of the Study:

    • To measure the recombination kinetics of CO to protoheme after laser-induced dissociation.
    • To investigate the influence of temperature and solvent viscosity on these recombination dynamics.
    • To determine the geminate recombination rate constant and characterize the escape fraction.

    Main Methods:

    • Utilized 25-ns laser photolysis to induce CO dissociation from protoheme in glycerol-water solutions.
    • Measured recombination kinetics as a function of temperature and viscosity.
    • Employed a model incorporating a single barrier and diffusive escape to simulate observed kinetics.

    Main Results:

    • Calculated a geminate recombination rate constant of 3 x 10^9/s.
    • Observed that the recombination rate coefficient is only weakly dependent on temperature and viscosity.
    • Found that the fraction of CO molecules escaping geminate recombination is dependent on solvent viscosity.

    Conclusions:

    • The geminate recombination of CO to protoheme is a rapid process occurring on a picosecond timescale.
    • Solvent viscosity plays a significant role in determining the extent of ligand escape post-photolysis.
    • The proposed kinetic model effectively simulates the observed recombination and escape phenomena.

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