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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
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Circular RNA Expression Profiling by Microarray-A Technical and Practical Perspective.

Yanggu Shi1, Jindong Shang1

  • 1Arraystar Inc., 9430 Key West Avenue #128, Rockville, MD 20850, USA.

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|May 16, 2023
PubMed
Summary

Circular RNAs (circRNAs) offer unique advantages as biomarkers due to their stability and specificity. This review highlights circRNA microarrays as an effective tool for profiling circRNA expression in research and clinical settings.

Keywords:
circRNAcircRNA microarraycircRNA profilingcircRNA-seqcircular RNAmicroRNA sponge

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Area of Science:

  • Biochemistry and Molecular Biology
  • Genomics and Bioinformatics
  • Biomarker Discovery

Background:

  • Circular RNAs (circRNAs) are covalently closed RNA loops with distinct biochemical properties.
  • Emerging research reveals diverse biological functions and clinical relevance of circRNAs.
  • circRNAs show promise as superior biomarkers compared to linear RNAs due to their stability and specificity.

Purpose of the Study:

  • To review circRNA microarrays as a practical and effective technology for circRNA expression profiling.
  • To share experiences and highlight key findings from circRNA profiling studies.
  • To facilitate advances in understanding circRNA biology and applications.

Main Methods:

  • Review of circRNA microarray technology.
  • Analysis of data from circRNA expression profiling studies.
  • Discussion of practical considerations for biological and clinical research labs.

Main Results:

  • circRNA microarrays provide a practical and effective method for profiling circRNA expression.
  • Profiling studies have yielded significant insights into circRNA biology and disease association.
  • circRNA expression patterns exhibit unique cell/tissue/disease specificities.

Conclusions:

  • circRNA microarrays are a valuable tool for advancing circRNA research.
  • Understanding circRNA expression is crucial for biomarker development and clinical applications.
  • The stability and specificity of circRNAs underscore their potential in diagnostics and therapeutics.