Synthesis and Application of Carbon Quantum Dots Derived from Carbon Black in Bioimaging

Carbon quantum dots (CQDs) are a new type of fluorescent QDs that consists mainly of carbon atoms. In this research, CQDs were synthesized through harsh oxidizing conditions applied on carbon black and subsequent N-doping using hexamethylenetetramine (Hexamine) and polyethyleneimine (PEI). The synthesized CQDs were characterized using FTIR, AFM, UV-Visible spectroscopy, photoluminescence (PL) spectroscopy, and fluorescence imaging respectively. The AFM images showed that the dots are in the range of 2-8 nm. N-doping of the CQDs increased the PL intensity. The PL enhancement for the CQDs that were N-doped with PEI was higher compared to those N-doped with hexamine. The shift in PL by changing the excitation wavelength has been attributed to the nano-size of the CQDs, functional groups, defect traps, and quantum confinement effect. The in vitro fluorescence imaging revealed that N-doped CQDs can internalize into the cells and be used for fluorescent cell imaging.