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Updated: Jul 30, 2025

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
Infrared Signatures of Phycobilins within the Phycocyanin 645 Complex
Partha Pratim Roy1,2, Cristina Leonardo1,2, Kaydren Orcutt1,2
1Department of Chemistry, University of California, Berkeley, California 94720, United States.
Abstract:
Aquatic photosynthetic organisms evolved to use a variety of light frequencies to perform photosynthesis. Phycobiliprotein phycocyanin 645 (PC645) is a light-harvesting complex in cryptophyte algae able to transfer the absorbed green solar light to other antennas with over 99% efficiency. The infrared signatures of the phycobilin pigments embedded in PC645 are difficult to access and could provide useful information to understand the mechanism behind the high efficiency of energy transfer in PC645. We use visible-pump IR-probe and two-dimensional electronic vibrational spectroscopy to study the dynamical evolution and assign the fingerprint mid-infrared signatures to each pigment in PC645. Here, we report the pigment-specific vibrational markers that enable us to track the spatial flow of excitation energy between the phycobilin pigment pairs. We speculate that two high-frequency modes (1588 and 1596 cm-1) are involved in the vibronic coupling leading to fast (
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