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Updated: Jul 30, 2025

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
Glycosylation Improves the Proteolytic Stability of Exenatide
Chaitra Chandrashekar1, Yuji Nishiuchi2,3, Barbara Fam White4
1The Florey Institute of Neuroscience and Mental Health, Parkville, Victoria 3052, Australia.
Abstract:
Exenatide was the first marketed GLP-1 receptor agonist for the treatment of type 2 diabetes. Modification to the chemical structure or the formulation has the potential to increase the stability of exenatide. We introduced human complex-type sialyloligosaccharide to exenatide at the native Asn28 position. The synthesis was achieved using both solid phase peptide synthesis (SPPS) and Omniligase-1-mediated chemoenzymatic ligation. The results demonstrate that glycosylation increases the proteolytic stability of exenatide while retaining its full biological activity.
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