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Related Experiment Videos

Intracellular recordings from salamander olfactory supporting cells.

D Trotier, P MacLeod

    Brain Research
    |May 28, 1986
    PubMed
    Summary

    Salamander olfactory supporting cells exhibit unique electrical properties, responding to odorants with delayed, reversed-polarity depolarizations. These findings suggest auxiliary roles in olfactory signal processing.

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    Area of Science:

    • Neuroscience
    • Olfactory System Physiology

    Background:

    • Supporting cells in the olfactory epithelium are crucial for olfactory receptor neuron function.
    • Their specific electrophysiological properties and responses to olfactory stimuli remain incompletely understood.

    Purpose of the Study:

    • To investigate the electrophysiological characteristics of salamander olfactory supporting cells.
    • To determine how these cells respond to olfactory nerve stimulation and odorant exposure.

    Main Methods:

    • Stable intracellular recordings were performed in the olfactory epithelium of salamanders.
    • Cells were recorded in the zone of highest supporting cell density.
    • Electrophysiological properties, including resting potential and input resistance, were measured.
    • Responses to changes in extracellular potassium and olfactory nerve stimulation were assessed.
    • Reactions to odorant stimulation were compared with the electro-olfactogram.

    Main Results:

    • Cells exhibited high resting potentials (-96 mV) and low input resistance, with no spontaneous or evoked spike activity.
    • Depolarization occurred with increased extracellular potassium.
    • Antidromic stimulation of olfactory axons induced rapid and slow depolarizations.
    • Odorant stimulation elicited graded depolarizations with a >1s delay compared to the electro-olfactogram.
    • These odorant-induced responses were consistent across cells, with similar amplitude but reversed polarity to the electro-olfactogram.

    Conclusions:

    • Salamander olfactory supporting cells possess distinct electrophysiological profiles.
    • They exhibit delayed, reversed-polarity depolarizations in response to odorants, distinct from olfactory receptor neurons.
    • These findings support a potential auxiliary role for supporting cells in olfactory signal modulation or processing.

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