Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-functional copper(I) halides with solvent-induced rapid luminescence switching toward information encryption and white-light emission.

Chemical communications (Cambridge, England)·2026
Same author

Aniline Degradation via 2-Aminophenol: Genetic Basis, Broadened Players and Interspecies Cooperation.

Environmental microbiology·2026
Same author

Unlocking the potential in municipal reclaimed water electrolysis for hydrogen production: Identification of the primary water matrix.

Water research·2026
Same author

Research on the impact of green dynamic capability and corporate green transformation-based on the moderating role of ESG management.

Scientific reports·2026
Same author

Steady-State and Dynamic Behavior of Geometry-Tunable Microfluidic Passive Flow Regulators.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

An intraocular oxygenated emulsion suppresses retinal fibrosis by inhibiting hypoxia-driven bioenergetic shifts and mesenchymal transformation.

Research square·2026

Related Experiment Video

Updated: Jul 30, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.8K

Dispersive phase microscopy incorporated with droplet-based microfluidics for biofactory-on-a-chip.

Yingdong Luo1, Yuanyuan Huang1, Yani Li1

  • 1A School of Mechanical Engineering and Electronic Information, China University of Geosciences, Wuhan, 430074, China. songcl@cug.edu.cn.

Lab on a Chip
|May 17, 2023
PubMed
Summary

This study introduces a novel microfluidic system for high-throughput single-cell phenotyping. It enables precise screening of cells based on intracellular biomolecules, advancing metabolic engineering and biofactory applications.

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.3K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

965

Related Experiment Videos

Last Updated: Jul 30, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.8K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.3K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

965

Area of Science:

  • Biotechnology and Metabolic Engineering
  • Microfluidics and Single-Cell Analysis

Background:

  • Current cell phenotyping methods are limited to population-scale analysis, hindering precise strain development.
  • Metabolic engineering requires high-throughput screening of single cells for desired phenotypes.

Purpose of the Study:

  • To develop a high-throughput single-cell phenotyping and screening system.
  • To enable precise identification and selection of cells based on intracellular biomolecule content.

Main Methods:

  • Integration of dispersive phase microscopy with droplet-based microfluidics.
  • Utilizing droplet volume-on-demand generation, biomolecular imaging, and droplet-on-demand sorting.
  • Encapsulating single cells in homogeneous microfluidic droplets for analysis.

Main Results:

  • Demonstrated high-throughput single-cell imaging and phenotype-based sorting.
  • Successfully applied the system to evolve *Haematococcus lacustris* for enhanced astaxanthin production.
  • Validated the system's potential for single-cell biomass quantification and selection.

Conclusions:

  • The proposed microfluidic system offers a powerful tool for high-throughput single-cell phenotyping and selection.
  • This technology has broad applicability in metabolic engineering, biofuel production, and cell therapy quality control.
  • Enables precise screening of cells based on intracellular metabolite biomass.