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Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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GlycoNote with Iterative Decoy Searching and Open-Search Component Analysis for High-Throughput and Reliable Glycan

Ming-Qi Liu^1,2, Guy Treves^2,3, Matthew Amicucci^2,3

¹Shanghai Fifth People's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.

Analytical Chemistry

|May 17, 2023

Summary

This summary is machine-generated.

GlycoNote is a new tool for interpreting mass spectrometry glycan data, improving the reliability and throughput of glycomic analysis. This generic platform enhances the broad usability of glycomics research for various sample types.

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Area of Science:

Glycomics
Mass Spectrometry
Glycobiology

Background:

Mass spectrometry-based glycome analysis is crucial for studying glycosylation.
Current tools lack high-throughput and reliability for glycan spectral interpretation, hindering glycomic research.
A need exists for generic, precise glycomic analysis tools.

Purpose of the Study:

To develop GlycoNote, a generic and reliable tool for comprehensive glycome analysis.
To enable high-throughput and precise interpretation of tandem-mass spectrometry glycomic data.
To facilitate broad usability of glycomic research across diverse sample sources.

Main Methods:

Development of GlycoNote, a novel glycomic analysis tool.
Implementation of a target-decoy method with iterative decoy searching for reliability.
Inclusion of an open-search component analysis mode for heterogeneity analysis.

Main Results:

GlycoNote demonstrated high capacity for glycome analysis across diverse datasets (human milk oligosaccharides, N-/O-glycomes, plant polysaccharides, C. elegans glycans).
Successful application to labeled and derived glycans confirmed its broad usability.
The tool provides generic characterization of various glycan types and elucidates component heterogeneity.

Conclusions:

GlycoNote is a freely available, promising tool for advancing glycomics.
It enhances the reliability and throughput of glycan spectral interpretation.
Facilitates comprehensive glycome analysis and heterogeneity elucidation in glycobiology research.