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CircPSMC3 Inhibits Colorectal Cancer Cell Growth and Migration by Controlling miR-31-5p/YAP/β-Catenin

Ye Jin1, Lingli Yu2, Qi Wu1

  • 1Department of General Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China.

Protein and Peptide Letters
|May 17, 2023
PubMed
Abstract

Insights

Circular RNA CircPSMC3 suppresses colorectal cancer (CRC) progression by inhibiting cell proliferation, invasion, and migration. It acts as a sponge for miR-31-5p, modulating the YAP/β-catenin pathway, suggesting CircPSMC3 as a potential CRC therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) is a significant global health concern.
  • Circular RNAs (CircRNAs) play crucial roles in cancer development.
  • The specific function of CircPSMC3 in CRC remains largely unelucidated.

Purpose of the Study:

  • To investigate the regulatory role of CircPSMC3 in colorectal cancer.
  • To explore the underlying molecular mechanisms of CircPSMC3 in CRC progression.
  • To evaluate CircPSMC3 as a potential therapeutic candidate for CRC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for gene expression analysis.
  • Cell proliferation assays (CCK-8, EdU) and western blotting for protein expression.
  • Cell invasion and migration assays (Transwell, wound healing) and luciferase reporter assays.

Main Results:

  • CircPSMC3 expression was significantly downregulated in CRC tissues and cell lines.
  • CircPSMC3 overexpression suppressed CRC cell proliferation, invasion, and migration.
  • CircPSMC3 directly targeted miR-31-5p, modulating the YAP/β-catenin signaling pathway.

Conclusions:

  • CircPSMC3 inhibits CRC cell growth and metastasis by sponging miR-31-5p and regulating the YAP/β-catenin axis.
  • These findings highlight CircPSMC3 as a potential therapeutic biomarker and target for colorectal cancer treatment.
Keywords:
CircPSMC3YAP/β-catenincolorectal cancermiR-31-5ppotential regulatory effectstissues.

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