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Negative Regulation of Innate Immune Signaling by Components of the Button Mushroom Agaricus bisporus

Michele O'Sullivan1, Elaine Dempsey2, Sarah Case1

  • 1School of Biochemistry & Immunology, Trinity College, Dublin 2, D02 R590, Ireland.

Molecular Nutrition & Food Research
|May 17, 2023
PubMed
Abstract

Insights

Mushroom powders from Agaricus bisporus can dampen immune responses by blocking innate immune receptors. This anti-inflammatory effect, observed in vitro and in vivo, suggests potential for managing chronic inflammation.

Area of Science:

  • Immunology
  • Mycology
  • Food Science

Background:

  • Mushrooms have a long history of use as food and medicine.
  • Mushroom components interact with the innate immune system, particularly macrophages.
  • Understanding mushroom-immune interactions is crucial for their health benefits.

Purpose of the Study:

  • To investigate the immunomodulatory effects of Agaricus bisporus mushroom powder.
  • To explore the potential of mushroom compounds in modulating innate immune signaling.
  • To assess the anti-inflammatory properties of mushroom powder in vitro and in vivo.

Main Methods:

  • Macrophages were pre-treated with Agaricus bisporus powder before stimulation with microbial ligands (LPS, β-glucans).
  • NFκB activation and pro-inflammatory cytokine production were measured.
  • The effect of simulated digestion on mushroom powder activity was assessed.
  • Colitis was induced in a DSS-mouse model to evaluate in vivo effects.

Main Results:

  • Mushroom powder pre-treatment attenuated innate immune signaling, including NFκB activation and cytokine production.
  • This inhibitory effect was observed at lower doses of microbial ligands, suggesting competitive inhibition.
  • The immunomodulatory effect was retained after simulated digestion.
  • In vivo administration of mushroom powder reduced colitis severity in mice.

Conclusions:

  • Agaricus bisporus mushroom powder exhibits significant anti-inflammatory properties.
  • Mushroom compounds may act by competitively inhibiting innate immune receptors.
  • These findings support the development of mushroom-based strategies for chronic inflammation management.
Keywords:
Dectin-1inflammationmacrophagesmushroomsβ-glucan

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