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The RUBY reporter enables efficient haploid identification in maize and tomato.

Dong Wang1, Yu Zhong1, Bin Feng1

  • 1National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.

Plant Biotechnology Journal
|May 17, 2023
PubMed
Summary

A new RUBY reporter system enables efficient visual identification of haploids in maize and tomato. This background-independent marker aids accurate haploid identification, crucial for doubled haploid breeding across diverse crop species.

Keywords:
RUBYBetalainDoubled haploidHaploid identificationMaizeTomato

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Area of Science:

  • Plant Science
  • Genetics
  • Agricultural Biotechnology

Background:

  • Haploid induction is vital for doubled haploid technology in various crops.
  • Current haploid identification methods (e.g., R1-nj, RFP, eGFP) have limitations in species specificity or equipment requirements.
  • An efficient, broadly applicable visual marker for haploid identification is needed.

Purpose of the Study:

  • To introduce and evaluate the RUBY reporter system as a novel visual marker for haploid identification in maize and tomato.
  • To assess the efficiency and accuracy of the RUBY reporter across different crop species.

Main Methods:

  • The RUBY reporter, a betalain biosynthesis system, was integrated into maize and tomato haploid inducers.
  • Expression of the RUBY reporter was analyzed for pigmentation in haploid embryos and seedlings.
  • Identification accuracy and timing were evaluated.

Main Results:

  • RUBY expression resulted in deep betalain pigmentation in maize embryos by 10 days post-pollination, enabling 100% accurate identification.
  • In tomato, RUBY induced deep red pigmentation in radicles, allowing easy and accurate haploid identification.
  • The RUBY marker demonstrated background independence.

Conclusions:

  • The RUBY reporter is an efficient and broadly applicable visual marker for haploid identification.
  • This marker system is promising for advancing doubled haploid breeding programs in diverse crop species.