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Updated: Jul 30, 2025
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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
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Multi-sample/multi-nucleus parallel polarization and monitoring enabled by a fluid path technology compatible
Thanh Phong Lê1, Jean-Noël Hyacinthe1,2,3, Andrea Capozzi4,5
1LIFMET, Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015, Lausanne, Switzerland.
Scientific Reports
|May 17, 2023
Summary
This study introduces a new cryogenic probe for dissolution Dynamic Nuclear Polarization (dDNP), enabling simultaneous hyperpolarization of three samples. This breakthrough significantly reduces sample preparation time for advanced preclinical and clinical applications.
Area of Science:
- Nuclear Magnetic Resonance Spectroscopy
- Hyperpolarization Techniques
- Preclinical Imaging
Background:
- Dissolution Dynamic Nuclear Polarization (dDNP) is limited by low throughput, requiring hours for single sample preparation.
- This bottleneck restricts the clinical and preclinical applications of hyperpolarized (HP) agents, particularly for direct 13C nuclei polarization.
- Increased sample throughput is crucial for expanding the scope and complexity of HP applications.
Purpose of the Study:
- To design and evaluate a versatile, customizable dDNP cryogenic probe for simultaneous multi-sample hyperpolarization.
- To assess the probe's capability for independent monitoring of solid-state spin dynamics for each sample.
- To demonstrate multi-nucleus NMR and in vivo application of the developed system.
Main Methods:
- Adaptation of a dDNP cryogenic probe to a 5 T preclinical polarizer, accommodating up to three samples.
- Independent monitoring of solid-state spin dynamics for each sample, irrespective of radical or nuclear species.
- Testing multi-nucleus polarization (13C, 1H, 129Xe) and in vivo HP Magnetic Resonance Spectroscopy (MRS) in a mouse model.
Main Results:
- The system successfully hyperpolarized three samples simultaneously within 30 minutes, achieving high repeatability (30.0 ± 1.2% 13C polarization for [1-13C]pyruvic acid).
- Demonstrated simultaneous multi-nucleus (13C, 1H, 129Xe) polarization and monitoring.
- Successfully implemented multi-substrate HP-MRS in vivo using [1-13C]lactate/[1-13C]pyruvate in a mouse model at 14.1 T.
Conclusions:
- The developed dDNP cryogenic probe significantly enhances throughput for hyperpolarized agent preparation.
- The system's versatility allows for independent sample monitoring and multi-nucleus capabilities.
- This advancement facilitates complex preclinical studies and holds promise for broader clinical translation of HP techniques.
