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Updated: Jul 30, 2025

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds
Published on: January 23, 2018
Diterpenoids and Their Glycosides from the Stems of Tinospora crispa with Beta-Cell Protective Activity
Sio-Hong Lam1, Hui-Kang Liu2,3, Shih-Yuan Chung4,5
1School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
Abstract:
Seven previously undescribed diterpenoids, tinocrisposides A-D (1-4) and borapetic acids A (5), B (6), and C (7), together with 16 known compounds, were isolated from the stem of Tinospora crispa (Menispermaceae). The structures of the new isolates were elucidated by spectroscopic and chemical methods. The β-cell protective effect of the tested compounds was examined on insulin-secreting BRIN-BD11 cells under dexamethasone treatment. Diterpene glycosides 12, 14-16, and 18 presented a substantial protective effect on BRIN-BD11 cells treated with dexamethasone in a dose-dependent manner. Compounds 4 and 17 with two sugar moieties exhibited clear protective effects on β-cells.
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