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Updated: Jul 30, 2025
05:23
Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
440
Gastric microbiome changes in relation with Helicobacter pylori resistance
Astri Dewayani1,2,3, Kartika Afrida Fauzia3,4,5, Ricky Indra Alfaray3,4
1Oita University Faculty of Medicine, Department of Infectious Disease Control, Yufu, Oita, Japan.
Plos One
|May 18, 2023
Summary
Multidrug-resistant Helicobacter pylori (H. pylori) infections increase stomach microbiome diversity and alter bacterial abundance. This suggests a link between H. pylori resistance and a less healthy gut environment.
Area of Science:
- Microbiology
- Gastroenterology
- Genetics
Background:
- Inadequate antimicrobial treatment drives multidrug-resistant (MDR) bacteria, including the stomach pathogen Helicobacter pylori (H. pylori).
- Antibiotic-induced alterations in the stomach microbiota can negatively impact host health.
- Understanding the relationship between H. pylori resistance and the stomach microbiome is crucial for effective treatment strategies.
Purpose of the Study:
- To investigate the influence of H. pylori antibiotic resistance on the diversity and abundance of the stomach microbiome.
- To compare the stomach microbiome composition in H. pylori-sensitive versus H. pylori-resistant (single, double, and triple resistance) patient groups.
Main Methods:
- Bacterial DNA was extracted from H. pylori-positive patient biopsy samples.
- 16S rRNA gene sequencing (V3-V4 regions) was performed to analyze microbiome community structure.
- In-vitro antibiotic susceptibility testing (E-test) determined resistance profiles; diversity and abundance were assessed.
Main Results:
- Multidrug-resistant H. pylori samples exhibited significantly elevated alpha-diversity parameters compared to sensitive samples (P <0.05).
- Triple-resistant H. pylori samples showed reduced Helicobacter abundance and increased Streptococcus abundance.
- LEfSe analysis identified specific bacterial genera associated with single and triple resistance profiles.
Conclusions:
- H. pylori resistance is associated with increased stomach microbiome diversity and evenness.
- Decreased H. pylori abundance in triple-resistant samples correlates with increased pathogenic bacteria, potentially promoting antimicrobial resistance.
- E-test antibiotic susceptibility may not fully capture the complex resistance status in vivo.
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