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Dual-modular immunosensor for bongkrekic acid detection using specific monoclonal antibody.
Xue-Ming Cao1, Li-Hua Li2, Hong-Zhi Liang3
1Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Journal of Hazardous Materials
|May 18, 2023
Summary
A new dual-modular immunosensor effectively detects Bongkrekic acid (BA), a dangerous food toxin. This tool uses a novel monoclonal antibody for sensitive and accurate on-site and lab testing of BA contamination in food.
Area of Science:
- Food Safety
- Analytical Chemistry
- Immunotechnology
Background:
- Bongkrekic acid (BA) is a highly lethal mitochondrial toxin frequently misdiagnosed.
- Accurate immunoassay for BA is hindered by the lack of specific antibodies.
Purpose of the Study:
- To develop a specific monoclonal antibody for Bongkrekic acid.
- To establish a dual-modular immunosensor for sensitive on-site and laboratory detection of BA.
Main Methods:
- Generation of a monoclonal antibody specific to BA.
- Development of a dual-modular immunosensor combining fluorescence assay (FA) and colorimetric assay (CA).
- Utilizing carbon dots (CDs) and oxidized TMB for FA, and gold nanostars (Au NSs) for CA.
Main Results:
- The monoclonal antibody demonstrated high affinity (Kd=0.33 μM) and sensitivity (IC50 =17.9 ng/mL) with minimal cross-reactivity.
- The dual-modular immunosensor achieved a limit of detection below 10 ng/mL for BA in food samples.
- Recovery rates ranged from 80.0% to 103.6%, consistent with LC-MS/MS analysis.
Conclusions:
- A novel monoclonal antibody and a dual-modular immunosensor were successfully developed for BA detection.
- The proposed immunosensor offers a sensitive, effective, and reproducible method for screening BA contamination in food.
- This tool addresses the challenge of BA immunoassay, improving food safety diagnostics.
