Testing the Effects of Magnetic Hyperthermia in 2D Cell Culture

Magnetic hyperthermia is an innovative thermal therapy for the treatment of solid malignancies. This treatment approach utilizes magnetic nanoparticles that are stimulated by alternating magnetic fields to induce temperature elevations in tumor tissue, resulting in cell death. Magnetic hyperthermia is clinically approved for treating glioblastoma in Europe and is undergoing clinical evaluation for prostate cancer in the United States. Numerous studies have also demonstrated efficacy in other cancers, however, and its potential utility extends far beyond its current clinical indications. Despite this great promise, assessing the initial efficacy of magnetic hyperthermia in vitro is a complicated endeavor, with multiple hurdles worth considering, such as accurate thermal monitoring, accounting for nanoparticle interference, and a myriad of treatment controls that make robust experimental planning essential to evaluate treatment outcome. Presented here is an optimized magnetic hyperthermia treatment protocol to test the primary mechanism of cell death in vitro. This protocol can be applied to any cell line and ensures accurate temperature measurements, minimal nanoparticle interference, and controls for multiple factors that can influence experimental outcome.