Thermalbibacter longus gen. nov., sp. nov., isolated from a hot spring

An isolate, designated CFH 74404^T, was recovered from a hot spring in Tengchong, Yunnan province, PR China. Phylogenetic analysis indicated that the isolate belongs to the family Thermomicrobiaceae and showed the highest 16S rRNA gene sequence similarity to Thermorudis peleae KI4^T (93.6 %), Thermorudis pharmacophila WKT50.2^T (93.1 %), Thermomicrobium roseum DSM 5159^T (92.0 %) and Thermomicrobium carboxidum KI3^T (91.7 %). The average amino acid identity and average nucleotide identity values between strain CFH 74404^T and the closest relatives were 42.0-75.9 % and 67.0-77.3 %, respectively. Cells of strain CFH 74404^T stained Gram-positive and were aerobic, non-motile and short rod-shaped. Growth occurred at 20-65 °C (optimum, 55 °C), pH 6.0-8.0 (optimum, pH 7.0) and with up to 2.0 % (w/v) NaCl (optimum 0-1.0 %, w/v). The predominant respiratory quinone was MK-8. The major fatty acids (>10 %) were C_18 : 0 (50.8 %) and C_20 : 0 (16.8 %). The polar lipid profile of strain CFH 74404^T included diphosphatidylglycerol, four unidentified phosphoglycolipids, phosphatidylinositol and three unidentified glycolipids. The G+C content of the genomic DNA was determined to be 67.1 mol% based on the draft genome sequence. On the basis of phenotypic, phylogenetic and genotypic analyses, it is concluded that strain CFH 74404^T represents a new species of a novel genus Thermalbibacter of the family Thermomicrobiaceae, for which the name Thermalbibacter longus gen. nov., sp. nov. is proposed. The type strain is CFH 74404^T (=KCTC 62930^T=CGMCC 1.61585^T).