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Performance of Chromogenic Protein C (PC) Testing.

Rebecca Adams1, Robyn Coleman1, Timothy Stanton2

  • 1Department of Haematology, Sullivan Nicolaides Pathology, Bowen Hills, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2023
PubMed
Summary

This study details a chromogenic assay for measuring functional Protein C (PC), a key regulator of blood coagulation. Accurate PC measurement is vital for diagnosing and managing thrombotic disorders like venous thromboembolism (VTE).

Keywords:
Antigenic assayChromogenic assayClotting-based assayProtein CThrombophiliaVenous thromboembolism

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Area of Science:

  • Biochemistry and Molecular Biology
  • Hematology
  • Clinical Diagnostics

Background:

  • Protein C (PC) is a crucial vitamin K-dependent protein synthesized in the liver, essential for regulating the blood coagulation cascade.
  • Activated PC (APC) inhibits thrombin generation by inactivating Factors Va and VIIIa, playing a vital role in preventing thrombosis.
  • PC deficiency is linked to an increased risk of venous thromboembolism (VTE) and severe fetal complications like purpura fulminans and DIC.

Purpose of the Study:

  • To describe a chromogenic assay for quantifying functional Protein C (PC) levels in plasma.
  • To provide a reliable method for assessing PC activity, important for diagnosing coagulation disorders.
  • To highlight the clinical significance of PC measurement in the context of VTE and bleeding disorders.

Main Methods:

  • The study details a chromogenic assay protocol for measuring functional PC.
  • This assay utilizes a PC activator, with color change intensity directly proportional to the amount of functional PC present.
  • Comparison is made to other available methods like functional clotting-based and antigenic assays, though not detailed.

Main Results:

  • The chromogenic assay quantitates the amount of functional PC in a plasma sample.
  • The degree of color change serves as a direct measure of PC activity.
  • This method offers a quantitative assessment of functional PC relevant for clinical diagnostics.

Conclusions:

  • The described chromogenic assay provides a quantitative measure of functional Protein C.
  • Accurate measurement of PC is critical for the diagnosis and management of thrombotic conditions.
  • This assay serves as a valuable tool in the investigation of coagulation disorders, particularly VTE.