Assessment of Platelet Function by Automated Light Transmission Aggregometry

Area of Science:

• Clinical diagnostics
• Hematology
• Hemostasis and thrombosis

Background:

• Light transmission aggregometry (LTA) is the established gold standard for platelet function testing.
• LTA is typically confined to specialized laboratories due to its manual and labor-intensive nature.
• Automated testing offers enhanced standardization and accessibility for platelet function analysis.

Purpose of the Study:

• To describe platelet aggregation measurement using Sysmex CS-Series and CN-Series routine blood coagulation analyzers.
• To outline the methodological differences between the CS-5100 and CN-6000 analyzers for platelet function testing.

Main Methods:

• Platelet aggregation was measured on the Sysmex CS-5100 and CN-6000 analyzers.
• CS-5100 involves manual pipetting for concentrated agonist preparation, with final dilution within the analyzer.
• CN-6000 utilizes an auto-dilution feature for automatic preparation of agonist dilutions and working concentrations.

Main Results:

• Both CS-Series and CN-Series analyzers enable platelet aggregation measurement.
• The CS-5100 requires manual preparation of concentrated agonists.
• The CN-6000 automates agonist dilution and preparation.

Conclusions:

• Sysmex CS-Series and CN-Series analyzers provide automated platforms for platelet function testing.
• These automated systems facilitate the performance of platelet aggregation tests in routine laboratory settings.
• Methodological differences exist in agonist preparation between the CS-5100 and CN-6000 analyzers.