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Lactate and Immunomagnetic-purified iPSC-derived Cardiomyocytes Generate Comparable Engineered Cardiac Tissue
Biorxiv : the Preprint Server for Biology
|May 19, 2023
Summary
Lactate purification of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) does not alter engineered cardiac tissue (ECT) properties compared to MACS purification. This finding supports the use of lactate purification for generating comparable hiPSC-ECT models for cardiac research.
Area of Science:
- Cardiovascular Biology
- Stem Cell Biology
- Biomaterials Science
Background:
- Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are crucial for studying cardiac diseases.
- Metabolic (lactate) purification of hiPSC-CMs has been questioned for potentially inducing an ischemic cardiomyopathy-like phenotype.
- Magnetic antibody-based cell sorting (MACS) is an alternative purification method.
Conclusions:
- Lactate purification and MACS purification yield hiPSC-CMs that generate hiPSC-ECTs with similar molecular and functional characteristics.
- Lactate purification does not appear to induce an irreversible detrimental change in hiPSC-CM phenotype for ECT generation.
