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Related Experiment Videos

Higher order structure in ribosomal RNA.

R R Gutell, H F Noller, C R Woese

    The EMBO Journal
    |May 1, 1986
    PubMed
    Summary

    Comparative sequence analysis reveals tertiary structure in 16S ribosomal RNAs (rRNAs). This study confirms a specific interaction between positions 570 and 866 in 16S rRNA, proving its phylogenetic significance.

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    Insect molecular biology·2006

    Area of Science:

    • Molecular Biology
    • Bioinformatics
    • Genetics

    Background:

    • Determining higher-order structure in large ribosomal RNAs (rRNAs) is crucial for understanding their function.
    • Comparative sequence analysis is the primary method for elucidating rRNA tertiary structures.
    • Previous studies have focused on secondary structures, leaving higher-order interactions less defined.

    Purpose of the Study:

    • To reveal the tertiary structure of 16S-like ribosomal RNAs (rRNAs).
    • To experimentally test a computer-predicted higher-order interaction within the 16S rRNA.
    • To confirm phylogenetically a specific base-pairing interaction.

    Main Methods:

    • Applied comparative sequence analysis to large ribosomal RNAs.
    • Utilized computer-generated predictions of potential higher-order interactions.
    • Selected organisms based on oligonucleotide catalog information for sequence analysis.
    • Verified predicted base-pairing through phylogenetic sequence evidence.

    Main Results:

    • Identified a specific higher-order interaction in 16S rRNA involving positions 570 and 866.
    • Confirmed a one-to-one Watson-Crick base-pairing correspondence between these positions.
    • Phylogenetically validated the interaction across multiple independent organisms.

    Conclusions:

    • The interaction between positions 570 and 866 in 16S rRNA is phylogenetically proven.
    • Comparative sequence analysis is a reliable method for determining complex rRNA structures.
    • This finding contributes to a more complete understanding of 16S rRNA tertiary structure.

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