Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

675
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
675
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.9K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AbCVista: a deep learning framework for predicting antibody conformational ensembles.

Protein & cellĀ·2026
Same author

CB-Dock3: an enhanced web server for protein-ligand blind docking.

Nucleic acids researchĀ·2026
Same author

Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.

Nature cancerĀ·2026
Same author

Energy stress-induced PKCĪ¶ S-glutathionylation is essential for LKB1 cytoplasmic translocation and AMPK activation.

Life metabolismĀ·2025
Same author

ETS1 Expression in Diabetic Foot Ulcers: Implications for Fibroblast Phenotype and Wound Healing Through the PP2A/YAP Pathway.

Journal of inflammation researchĀ·2024
Same author

ConvNeXt-MHC: improving MHC-peptide affinity prediction by structure-derived degenerate coding and the ConvNeXt model.

Briefings in bioinformaticsĀ·2024

Related Experiment Video

Updated: Jul 29, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.5K

Abalign: a comprehensive multiple sequence alignment platform for B-cell receptor immune repertoires.

Fanjie Zong1,2, Chenyu Long1,2, Wanxin Hu1,2

  • 1Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Nucleic Acids Research
|May 19, 2023
PubMed
Summary

Abalign offers ultrafast multiple sequence alignment for B-cell receptor (BCR) sequencing data, significantly reducing analysis time. This tool enhances immunoinformatics research by providing efficient BCR analysis capabilities.

More Related Videos

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.3K
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

26.7K

Related Experiment Videos

Last Updated: Jul 29, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.5K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.3K
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

26.7K

Area of Science:

  • Immunoinformatics
  • Computational Biology
  • Adaptive Immunity

Background:

  • High-throughput sequencing (HTS) generates vast amounts of B-cell receptor (BCR) data for adaptive immunity and antibody development.
  • Current multiple sequence alignment (MSA) methods struggle with the scale of BCR sequencing data and lack immunoglobulin-specific insights.
  • Efficient analysis of large BCR datasets is crucial for advancing understanding of immune responses and drug discovery.

Purpose of the Study:

  • To develop a novel, ultrafast multiple sequence alignment (MSA) program specifically for B-cell receptor (BCR) and antibody sequences.
  • To address the computational challenges posed by the massive scale of HTS data in BCR immune repertoire analysis.
  • To provide a comprehensive and user-friendly tool for various BCR analysis tasks beyond basic alignment.

Main Methods:

  • Introduction of Abalign, a standalone software tool engineered for rapid MSA of BCR/antibody sequences.
  • Performance evaluation through benchmark tests comparing Abalign against existing state-of-the-art MSA tools.
  • Integration of a graphical user interface (GUI) for enhanced usability on personal computers.

Main Results:

  • Abalign demonstrates comparable or superior accuracy to current MSA tools.
  • Abalign significantly outperforms existing tools in speed and memory efficiency, reducing analysis time from weeks to hours.
  • The software successfully handles massive BCR sequencing datasets, enabling complex analyses like lineage tree construction and repertoire comparison.

Conclusions:

  • Abalign provides an effective and user-friendly solution for analyzing large-scale BCR and antibody sequence data.
  • The tool accelerates immunoinformatics research by overcoming computational bottlenecks in BCR repertoire analysis.
  • Abalign facilitates new discoveries in adaptive immunity and antibody drug development through efficient data processing and analysis.