One does not simply apply larvicides: Aedes aegypti from Araraquara (Brazil) has reduced susceptibility to pyriproxyfen

Using larvicides is an essential method in the strategy to control the Aedes aegypti (Diptera: Culicidae) mosquito in Brazil. However, over the years this practice can select resistant strains, decreasing the efficiency of the larvicide in vector control. We compared two Aedes aegypti populations to verify mosquito resistance to pyriproxyfen larvicide: a population from Araraquara and a susceptible Rockefeller strain. We tested four concentrations of pyriproxyfen (0.005, 0.01, 0.02, and 0.04 mg/L) and observed that the Araraquara strain showed a significant reduction in mortality when compared to the Rockefeller strain, except in the highest concentration tested. We also found a moderate resistance for the Araraquara larvae, which may be related to Araraquara temperatures due to be optimum for Ae. aegypti most part of the epidemic periods. Survivor mosquitoes of pyriproxyfen exposure showed reduced wing centroid sizes, which is related to the mosquitoes' vectorial capacity: frequency of blood meals, chances of hematophagy, and virus dissemination ability. Our results provide the current susceptibility status of an Araraquara Ae. aegypti population and can assist in promoting information to epidemiologic surveillance agencies.