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Updated: Jul 29, 2025
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Detection of β-lactoglobulin under different thermal-processing conditions by immunoassay based on nanobody and
Shijie Li1, Linqing Nie1, Yi Wang1
1Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
Abstract:
The problems of inaccurate detection values of thermal-processed β-lactoglobulin (β-LG) content seriously affect the screening of allergens. A monoclonal antibody (mAb) against β-LG was successfully prepared and a highly sensitive sandwich ELISA (sELISA) was constructed with specific nanobody (Nb) as the capture antibody with detection limit of 0.24 ng/mL. Based on this sELISA, the ability of Nb and mAb to recognize β-LG and β-LG interacting with milk components was explored. Combined with protein structure analysis to elaborate the mechanism of shielding β-LG antigen epitopes during thermal-processing, thus enabling the differentiation between pasteurized and ultra-high temperature sterilized milk, the detection of milk content in milk-containing beverages, and the highly sensitive detection and analysis of β-LG allergens in dairy-free products. The method provides methodological support for identifying the quality of dairy products and reducing the risk of β-LG contamination in dairy-free products.
