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Related Experiment Videos

Buffering capacity of human semen.

E Wolters-Everhardt, J M Dony, W A Lemmens

    Fertility and Sterility
    |July 1, 1986
    PubMed
    Summary

    Semen has a significantly higher buffering capacity than serum, important for male reproductive health. This capacity varies within individuals and does not correlate with fertility markers.

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    Area of Science:

    • Reproductive Biology
    • Biochemistry
    • Andrology

    Background:

    • Buffering capacity is crucial for maintaining physiological pH.
    • Semen's unique composition suggests distinct buffering properties compared to other bodily fluids.

    Purpose of the Study:

    • To determine the buffering capacity of human semen.
    • To compare semen buffering capacity with that of serum.
    • To investigate the variability and correlation of semen buffering capacity with fertility.

    Main Methods:

    • Titration curves were used to measure buffering capacity.
    • 270 semen samples from 196 men were analyzed.
    • Semen buffering capacity was compared to serum buffering capacity (n=42).

    Main Results:

    • Average semen buffering capacity (41.1 slyke) was significantly higher than serum (23.3 slyke) in the pH 7.0 to 6.0 range (P < 0.01).
    • Inter-individual variation in semen buffering capacity was greater than measurement error.
    • No correlation was observed between semen and serum buffering capacity in the same individual.
    • No correlation was found between semen buffering capacity and fertility grade.

    Conclusions:

    • Human semen possesses a robust buffering capacity, exceeding that of serum.
    • Semen buffering capacity is variable and not directly linked to male fertility status.
    • Further research may explore the specific components contributing to semen's buffering ability.

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