Effect of ethanol on proteolysis in isolated liver cells

Ethanol, when tested alone, inhibited proteolysis by about 20%; however, no effect was detected when it was combined with exogenous oxidizable fuels which inhibited proteolysis by themselves. Ethanol was effective in inhibiting proteolysis in the presence of protease inhibitors like ammonia, leupeptin or methylamine, indicating that its mechanism of action involves a non-lysosomal pathway of degradation. Ethanol oxidation is mandatory for it to have effect on proteolysis, however, its action is not related to an increased state of reduction of the NAD system. In contrast to other reductants of the NAD system, ethanol effect is accompanied by a rise in the phosphorylation state of the adenine nucleotides, suggesting that its action might be related to the cellular energy state.