A rapid diagnosis and treatment of Ornithonyssus bacoti infection

Area of Science:

• Veterinary Parasitology
• Molecular Identification of Parasites
• Medical Entomology

Background:

• Mites are significant pathogens and allergens affecting human and animal health.
• Morphological similarities among mite species complicate accurate identification and classification.
• An unusual skin condition in mice prompted an investigation into potential parasitic causes.

Purpose of the Study:

• To identify the mite species responsible for a skin condition in mice.
• To develop and validate an effective treatment strategy for the identified mite.
• To utilize molecular techniques for precise mite classification.

Main Methods:

• Morphological examination of the parasite.
• DNA extraction, PCR amplification, and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene.
• Phylogenetic analysis for species identification.
• Ivermectin gradient testing to determine optimal treatment concentration.

Main Results:

• The parasite was identified as Ornithonyssus bacoti-KF using morphological and molecular methods.
• Phylogenetic analysis confirmed the species identification.
• A 0.1 mg/mL ivermectin concentration in a bath was found to be the most effective treatment.
• No recurrence of the mite infestation was observed for six months post-treatment.

Conclusions:

• Ornithonyssus bacoti-KF is an uncommon mite that can cause significant health issues in laboratory mice.
• Microscopic examination combined with PCR and sequencing provides reliable identification of mite species.
• Ivermectin is an effective treatment for Ornithonyssus bacoti infestations, with a specific concentration recommended for optimal results.