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Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos
Fides Zenk1, Francesco Cardamone2,3, Dafne Andrea Ibarra Morales2,3
1Department of Biosystems Science and Engineering ETH (D-BSSE ETH Zürich), Basel, Switzerland. fides.zenk@bsse.ethz.ch.
Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2023
Abstract:
CUT&Tag is a method to map the genome-wide distribution of histone modifications and some chromatin-associated proteins. CUT&Tag relies on antibody-targeted chromatin tagmentation and can easily be scaled up or automatized. This protocol provides clear experimental guidelines and helpful considerations when planning and executing CUT&Tag experiments.

