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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
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Related Experiment Video

Updated: Jul 29, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Fluorescence Molecular Tomography for Quantum Yield and Lifetime.

Wenxiang Cong, Ge Wang

    Arxiv
    |May 22, 2023
    PubMed
    Summary

    This study introduces a novel fluorescence molecular tomography (FMT) algorithm combining time-resolved imaging with photon-counting micro-CT (PCMCT) for enhanced accuracy in reconstructing fluorescence yield and lifetime in mouse models.

    Area of Science:

    • Biomedical Imaging
    • Optical Imaging
    • Molecular Imaging

    Background:

    • Fluorescence molecular tomography (FMT) enables non-invasive imaging of internal fluorescence agents in biological tissues.
    • Applications span diagnosis, therapy, and drug design, particularly in small animal models.
    • Accurate reconstruction of fluorescence yield and lifetime is crucial for quantitative FMT.

    Approach:

    • A new fluorescent reconstruction algorithm is presented, integrating time-resolved fluorescence imaging data.
    • Photon-counting micro-CT (PCMCT) images are incorporated to provide prior knowledge of the permissible region of interest for fluorescence yield and lifetime.
    • This integration reduces unknown variables in the inverse problem, enhancing reconstruction stability.

    Key Points:

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  • The combined approach improves image reconstruction stability and accuracy.
  • Numerical simulations demonstrate the method's robustness against data noise.
  • An average relative error of 18% was achieved in reconstructing fluorescent yield and lifetime.

    • Conclusions:

    • The developed algorithm offers a more stable and accurate method for quantitative FMT.
    • Incorporating PCMCT data significantly enhances the reconstruction of fluorescence parameters.
    • This advancement holds potential for improved diagnostic and therapeutic applications using FMT.