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Related Concept Videos

RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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MicroRNAs01:22

MicroRNAs

21.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Related Experiment Video

Updated: Jul 29, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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MiSiPi-Rna: an integrated tool for characterizing small regulatory RNA processing.

Taiya Jarva1, Jialin Zhang2, Alex Flynt1

  • 1University of Southern Mississippi.

Biorxiv : the Preprint Server for Biology
|May 22, 2023
PubMed
Summary

Researchers can now easily analyze small RNA populations in new species using MiSiPi-Rna. This R package aids in understanding RNA interference (RNAi) biology and annotating microRNAs (miRNAs) and small-interfering RNAs (siRNAs).

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • RNA interference (RNAi) relies on small RNAs like microRNAs (miRNAs), small-interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs).
  • Investigating small RNA pathways in non-model organisms is crucial for advancing RNAi research and biotechnology.
  • Rapid evolution of small RNAs necessitates species-specific analysis before implementing RNAi strategies.

Conclusions:

  • MiSiPi-Rna addresses challenges in annotating global small RNA populations.
  • Aids researchers in efficiently studying species-specific RNAi mechanisms.
  • Supports the application of RNAi in biotechnology and non-model systems.