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Related Experiment Video

Updated: Jul 29, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Pupil engineering for extended depth-of-field imaging in a fluorescence miniscope.

Joseph Greene¹, Yujia Xue¹, Jeffrey Alido¹

¹Boston University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United States.

|May 22, 2023

Summary

We developed an extended depth-of-field (EDoF) miniscope by integrating a diffractive optical element (DOE) onto gradient refractive index (GRIN) lenses. This innovation significantly enhances neural population imaging depth and quality for neuroscience research.

Keywords:

computational imaging extended depth-of-field fluorescence microscopy miniscope

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Last Updated: Jul 29, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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Published on: August 15, 2014

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Area of Science:

Neuroscience
Optical Engineering
Biomedical Imaging

Background:

Miniscopes are vital for in-vivo neural population analysis.
High numerical aperture (NA) gradient refractive index (GRIN) lenses limit miniscope depth-of-field (DoF).

Purpose of the Study:

To develop an extended depth-of-field (EDoF) miniscope.
To overcome the DoF limitations of conventional miniscopes.

Main Methods:

Integrated a binary diffractive optical element (DOE) onto a GRIN lens.
Optimized DOE using a genetic algorithm considering GRIN lens aberrations and scattering.
Manufactured DOE via single-step photolithography.

Main Results:

Extended DoF by creating twin foci in scattering samples.
Achieved high-contrast signals without compromising speed, resolution, size, or weight.
Demonstrated deeper neural population interrogation in mouse brain samples.

Conclusions:

The low-cost EDoF-Miniscope is built from off-the-shelf components.
Customizable DOE allows for wide utility in neural recording.
This technology facilitates deeper and more comprehensive neural activity analysis.