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Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
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Jaceosidin inhibits the progression and metastasis of NSCLC by regulating miR-34c-3p/Integrin α2β1 axis

Qiao-Ru Guo1, Wen-Min Zhou1, Guo-Bin Zhang1

  • 1Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, China.

Heliyon
|May 22, 2023
PubMed

Insights

Jaceosidin, a flavonoid, boosts miR-34c-3p expression, suppressing non-small cell lung cancer (NSCLC) cell proliferation, migration, and invasion by targeting integrin α2β1.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Non-coding RNAs, including microRNAs (miRNAs), play significant roles in cancer development.
  • miR-34c-3p acts as a tumor suppressor in non-small cell lung cancer (NSCLC).
  • Identifying natural compounds that modulate miRNA expression is a promising therapeutic strategy.

Purpose of the Study:

  • To screen for flavonoids that up-regulate miR-34c-3p expression in NSCLC cells.
  • To evaluate the anticancer effects of identified flavonoids.
  • To elucidate the underlying molecular mechanism of action.

Main Methods:

  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) for gene expression analysis.
  • Cell proliferation assays (CCK-8, EdU).
  • Cell migration and invasion assays (wound healing, Transwell).

Main Results:

  • Jaceosidin significantly increased miR-34c-3p expression in A549 cells.
  • Jaceosidin inhibited proliferation, migration, and invasion of NSCLC cells (A549 and H1975) in a dose-dependent manner.
  • miR-34c-3p was found to target integrin α2β1, inhibiting its expression and consequently suppressing NSCLC cell migration and invasion.

Conclusions:

  • Jaceosidin exhibits significant anticancer activity against NSCLC.
  • The mechanism involves up-regulation of miR-34c-3p, which targets integrin α2β1.
  • Jaceosidin represents a potential therapeutic lead compound for NSCLC treatment.
Keywords:
Integrin α2β1InvasionJaceosidinMigrationNon-small cell lung cancer (NSCLC)miR-34c-3p

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