Chemical profiling of Cannabis sativa from eleven Tanzanian regions

The aim of this research was to investigate the chemical profiles of Cannabis sativa from 11 Tanzanian regions using preliminary tests as well as instrumental analyses with GC-MS and LC-MS. Generally, all the seized samples tested positive for the presence of (Δ⁹-THC. The preliminary test with Duquenois method followed by chloroform addition revealed the presence of Δ⁹-tetrahydrocannabinol (Δ⁹-THC) in all the samples. GC-MS analyses of the samples revealed the presence of nine cannabinoids including Δ⁹-THC, Δ⁸-THC, cannabidivarol, cannabidiol, Δ⁹-tetrahydrocannabivarin (Δ⁹-THCV), cannabichromene, cannabinol, caryophyllene, and cannabicouramaronone, whereas LC-MS chemical profiling revealed the presence 24 chemical substances, including 4 cannabinoids, 15 different types of drugs and 5 amino acids. The Pwani region had the highest percentage composition of Δ⁹-THC (13.45%), the main psychoactive ingredient of Cannabis sativa, followed by Arusha (10.92%) and Singida (10.08%). The sample from Kilimanjaro had the lowest percentage of Δ⁹-THC (6.72%). Apart from cannabinoids, the majority of other chemical substances were found in the Dar es Salaam region sample, which could be attributed to the fact that the city is the epicenter of business rather than the cultivation area, implying that the samples were obtained from different sources and blended as a single package.