Electrochemical H₂O₂ sensor based on a Au nanoflower-graphene composite for anticancer drug evaluation

Reliable H₂O₂ sensors for in situ cellular monitoring under drug stimulation can be developed as a powerful and versatile tool for drug evaluation. Herein, a novel electrochemical biosensor capable of detecting and quantifying H₂O₂ was fabricated by graphene and shape-controlled gold nanostructures. With the help of polyelectrolytes, gold exhibited hierarchical flower-like nanostructures. This kind of nanozyme material exhibited a prominent electrochemical response for H₂O₂. Electrocatalytic activity for H₂O₂ reduction with high sensitivity (5.07◊10^-4 mA μmol L^-1 cm^-2) and good detection capability (the lowest detection limit is 4.5 μmol L^-1 (S/N = 3)) were achieved. This electrochemical biosensor was successfully used to measure the concentration of H₂O₂ released from HepG2 hepatoma cells. Ascorbic acid (AA) and Camellia nitidissima Chi saponins (CNCS) were selected as model drugs, and their anticancer activities were compared by in situ monitoring of H₂O₂. Interestingly, the electrochemical sensor showed remarkable sensitivity, accuracy, and rapidity compared with the traditional enzymatic detection kit. In brief, the as-synthesized nanostructured H₂O₂ sensors can be applied to assess the antitumor properties of candidate drugs and inspire developments for personalized health care monitoring and cancer treatment.