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Conjunctival Commensal Isolation and Identification in Mice
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A comparative study on two methods of ocular surface microbial sampling.

Xinyi Shen1, Yi Xu1, Jinzhi Huang1

  • 1National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.

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|May 22, 2023
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Summary

A novel aerosolization ocular surface microorganism sampling method demonstrated higher accuracy and detected more microbes than traditional swab sampling. While promising, it complements rather than replaces existing methods for diagnosing ocular surface infections.

Keywords:
COVID-19Conjunctival sacCorneaInfection surveillance and controlMicrobial aerosolOcular surface microbial infectionSampling method

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Area of Science:

  • Ophthalmology
  • Microbiology
  • Diagnostic Techniques

Background:

  • Ocular surface infections require accurate microbial detection for effective treatment.
  • Traditional conjunctival sac swab sampling is a common method but may have limitations in sensitivity and scope.
  • Novel sampling techniques are being explored to improve the diagnosis of ocular microbial infections.

Purpose of the Study:

  • To compare the efficacy of a novel aerosolization ocular surface microorganism sampling method against traditional conjunctival sac swab sampling.
  • To evaluate the diagnostic accuracy, sensitivity, and specificity of both methods in detecting ocular microbial infections.

Main Methods:

  • The study involved 61 participants (122 eyes) undergoing both traditional swab sampling (Method A) and aerosolization sampling (Method B).
  • Aerosolization sampling involved generating aerosols from the ocular surface after air pulse impingement to capture microorganisms.
  • Samples were incubated and microbial identification was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Main Results:

  • The aerosolization method (Group B) showed higher accuracy (45.8% vs. 38.3%), sensitivity (57.1% vs. 35.7%), and specificity (44.3% vs. 38.7%) compared to swab sampling (Group A).
  • A significantly greater number of microbial types were detected using the aerosolization method (37 types) versus the swab method (12 types).
  • There was a low level of agreement between the two sampling methods (k=0.031).

Conclusions:

  • The novel aerosolization sampling method offers improved accuracy and broader microbial detection compared to traditional swab sampling for ocular surface infections.
  • While superior in certain aspects, the aerosolization method does not entirely replace swab sampling.
  • This technique presents a valuable supplementary strategy for the auxiliary diagnosis of ocular surface infections.