Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Diabetes Mellitus: Type 2 and Gestational

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...

Diabetes Mellitus: Overview and Type I Subtype

Diabetes Mellitus: Overview and Type I Subtype

Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative analysis of the m6A epitranscriptome in HeLa-expressed HPV18 early transcripts reveals its potential impact on splicing.

RNA (New York, N.Y.)·2026

Same author

The long noncoding RNA <i>lnc-FAM164A1</i>-ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach.

Frontiers in immunology·2026

Same author

Mitochondrial Extracellular Vesicles Enhance Aortic Valve Calcification Via Carnitine O-Octanoyltransferase-Brief Report.

Arteriosclerosis, thrombosis, and vascular biology·2026

Same author

Comparative Proteomic Analysis of Lipoprotein(a): Method-Dependent Profiles and Disease Pathways.

Journal of clinical medicine·2026

Same author

Editorial: Immunity, atherosclerosis, and cardiovascular disease: an interdisciplinary approach to cardiometabolic health.

Frontiers in cardiovascular medicine·2026

Same author

Impact of Variations in Commercial Standard Operating Procedure on Plasma Proteome Recovery.

Journal of proteome research·2026

Same journal

An Optimized RT-qPCR Protocol for Comprehensive Analysis of microRNAs and mRNAs in <i>Mus musculus</i> Brain Tissues.

Non-coding RNA·2026

Same journal

Investigation of Long Non-Coding RNAs <i>H19</i> rs3741219, <i>MEG3</i> rs7158663, <i>POLR2E</i> rs3787016, and <i>ANRIL</i> rs10757274 with Breast Cancer Susceptibility and Clinicopathological Characteristics in a Mexican Population.

Non-coding RNA·2026

Same journal

Comprehensive lincRNA Transcriptome in Acute Myeloid Leukemia: Integrating Known and Newly Identified lincRNAs Across Pediatric and Adult Cohorts.

Non-coding RNA·2026

Same journal

Exploratory Machine Learning Analysis of circRNA-Derived Molecular Features in Autism Spectrum Disorder.

Non-coding RNA·2026

Same journal

Urinary Exosomal microRNAs as a Novel Approach to Study People with Multiple Sclerosis and Severe Gait Disability: A Preliminary Observation.

Non-coding RNA·2026

Same journal

Hnf1aos1 as a Metabolic Coordinator of Hepatic Lipid Homeostasis and Feedback Control.

Non-coding RNA·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 29, 2025

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes.

Rebecca Distefano¹, Mirolyuba Ilieva², Jens Hedelund Madsen²

¹Section for Computational and RNA Biology, Department of Biology, University of Copenhagen, DK-2200 Copenhagen, Denmark.

|May 23, 2023

Summary

This summary is machine-generated.

This study analyzes long non-coding RNAs (lncRNAs) in type II diabetes (T2D) pathogenesis. We identified T2D-related lncRNAs and developed a web tool, T2DB, to aid future research.

Keywords:

RNA-seq database diabetes lncRNA

More Related Videos

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Related Experiment Videos

Last Updated: Jul 29, 2025

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Type II diabetes (T2D) is a global health concern linked to obesity and severe complications like cardiovascular and kidney diseases.
Understanding T2D pathogenesis is crucial for mitigating hyperglycemia's harmful effects.
Long non-coding RNAs (lncRNAs) are emerging as key players, yet remain understudied in T2D due to a focus on protein-coding genes in existing datasets.

Purpose of the Study:

To systematically analyze lncRNA expression changes in T2D patients using existing RNA sequencing data.
To investigate the functional role of a specific T2D-related lncRNA, USP30-AS1, in immune cell activation.
To create a centralized resource, T2DB, for lncRNA and protein-coding gene expression profiling in T2D.

Main Methods:

Secondary analysis of published RNA sequencing data from T2D patients and those with related complications.
Loss-of-function experiments using an in vitro model of pro-inflammatory macrophage activation to study USP30-AS1.
Development of the T2DB web application for expression profiling.

Main Results:

Systematic analysis revealed differential expression of lncRNAs in T2D patients compared to controls.
Functional experiments provided insights into the role of USP30-AS1 in macrophage activation.
The T2DB web application was successfully developed, offering a comprehensive resource for T2D-related gene expression.

Conclusions:

lncRNAs represent a significant, under-explored area in T2D pathogenesis.
USP30-AS1 is implicated in immune responses relevant to T2D.
The T2DB tool will accelerate lncRNA research in type II diabetes.