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Reference population database for 24 STR loci of selected provinces of Argentina
Mariela Caputo1,2, Andrea Sala3,4, Daniel Corach3,4
1Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Biotecnología, Inmunología y Genética. Catedra de Genética Forense, Centro de Referencia en Identificación Humana y Servicio de Huellas Digitales Genéticas (SHDG), Universidad de Buenos Aires, CABA, Argentina. mcaputo@ffyb.uba.ar.
Background:
Argentinean population is the result of admixture between South Amerindians, Europeans and to a lesser degree, Africans. Since the advent of forensic molecular genetics, the construction of local reference databases became mandatory. Aiming to further extend the technical quality reference database of Argentina, we present herein the allele frequencies for 24 autosomal STRs, including D22S1045, and SE33 (not previously reported for Argentina in STRidER).
Conclusions:
Genotypes of 6454 unrelated individuals (3761 males and 2694 females) from 13 out of 23 provinces were analysed. Forensic parameters were calculated for each marker. The observed heterozygosity ranged from 0.661 (TPOX) to 0.941 (SE33). The locus SE33 was revealed to be the most informative marker showing the highest values for PIC (0.955), GD (0.952), TPI (8.455) and PE (0.879). On the other hand, TPOX turned out to be the least informative marker: PIC (0.618), GD (0.669), and PE (0.371). The high number of analyzed individuals allowed detecting low frequency alleles and microvariants in CSF1PO; D16S539 and D21S11 D18S51; PENTA D; PENTA E and at locus D6S1043.
Methods And Results:
This study is the most extensive for Argentina and complements the already reported information concerning the autosomal STRs commonly used in forensic identification. The results were submitted passing STRidER quality control standards (QC), receiving the reference number STR000327 v.2.
